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《医学前沿(英文)》 2016年 第10卷 第4期 页码 517-521 doi: 10.1007/s11684-016-0480-9
This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients (70.41%). The susceptibility to triple antibiotic ointment (neomycin/bacitracin/polymyxin B) and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.
关键词: antimicrobial susceptibility/resistance Propionibacterium acnes topical antibiotics in vitro study
Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG
《农业科学与工程前沿(英文)》 2017年 第4卷 第1期 页码 106-115 doi: 10.15302/J-FASE-2017133
关键词: dehydroabietic acid-based diarylamine DFT study fluorescent probe in vitro imaging
Kaifeng RAO, Na LI, Mei MA, Zijian WANG
《环境科学与工程前沿(英文)》 2014年 第8卷 第1期 页码 69-78 doi: 10.1007/s11783-013-0502-7
关键词: waste water in vitro recombinant yeast assay ethoxyresorufin-O-deethylase (EROD) receptor endocrine
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《医学前沿(英文)》 2016年 第10卷 第1期 页码 41-51 doi: 10.1007/s11684-016-0429-z
Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme linked to tumor progression and a novel interacting partner of breast cancer 1, early-onset (BRCA1). However, the role of MID2 in breast cancer remains unknown. This study investigated the expression, prognostic value, and role of MID2 in breast cancer. The expression of MID2 mRNA and protein was significantly upregulated in breast cancer tissue and established cell lines compared with that in normal breast epithelial cells and paired adjacent non-tumor tissue (P<0.001). Immunohistochemical analysis demonstrated that MID2 was overexpressed in 272 of 284 (95.8%) paraffin-embedded, archived breast cancer tissue. Moreover, MID2 expression increased with advanced clinical stage (P<0.001). High MID2 expression was significantly associated with advanced clinical stages and T, N, and M staging (all P<0.05). Univariate and multivariate analyses indicated that high MID2 expression was an independent prognostic factor for poor overall survival in the entire cohort (93.73 vs. 172.1 months; P<0.001, log-rank test) and in subgroups with stages Tis+ I+ II and III+ IV. Furthermore, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide colony formation, and anchorage-independent growth ability assays were conducted. Results showed that siRNA silencing of MID2 expression significantly reduced MCF-7 and MDA-MB-231 cell proliferation in vitro and blocked the growth of MDA-MB-231 cell xenograft tumors in vivo (P<0.05). This study indicated that MID2 may be a novel prognostic marker and interventional target in breast cancer.
关键词: breast cancer MID2 proliferation overall survival xenograft
IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
《农业科学与工程前沿(英文)》 2021年 第8卷 第4期 页码 635-644 doi: 10.15302/J-FASE -2020343
The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.
关键词: Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis
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《医学前沿(英文)》 2018年 第12卷 第3期 页码 307-318 doi: 10.1007/s11684-017-0547-2
Acute ischemic stroke (AIS), as the third leading cause of death worldwide, is characterized by its high incidence, mortality rate, high incurred disability rate, and frequent reoccurrence. The neuroprotective effects of extract (GBE) against several cerebral diseases have been reported in previous studies, but the underlying mechanisms of action are still unclear. Using a novel rat cortical capillary endothelial cell-astrocyte-neuron network model, we investigated the neuroprotective effects of GBE and one of its important constituents, Ginkgolide B (GB), against oxygen–glucose deprivation/reoxygenation and glucose (OGD/R) injury. In this model, rat cortical capillary endothelial cells, astrocytes, and neurons were cocultured so that they could be synchronously observed in the same system. Pretreatment with GBE or GB increased the neuron cell viability, ameliorated cell injury, and inhibited the cell apoptotic rate through Bax and Bcl-2 expression regulation after OGD/R injury. Furthermore, GBE or GB pretreatment enhanced the transendothelial electrical resistance of capillary endothelial monolayers, reduced the endothelial permeability coefficients for sodium fluorescein (Na-F), and increased the expression levels of tight junction proteins, namely, ZO-1 and occludin, in endothelial cells. Results demonstrated the preventive effects of GBE on neuronal cell death and enhancement of the function of brain capillary endothelial monolayers after OGD/R injury ; thus, GBE could be used as an effective neuroprotective agent for AIS/reperfusion, with GB as one of its significant constituents.
关键词: acute ischemic stroke Ginkgo bilobaextract Ginkgolide B network model neuroprotection
Biodegradable polymethacrylic acid grafted psyllium for controlled drug delivery systems
Ranvijay KUMAR, Kaushlendra SHARMA
《化学科学与工程前沿(英文)》 2013年 第7卷 第1期 页码 116-122 doi: 10.1007/s11705-013-1310-0
关键词: psyllium acetyl salicylic acid in-vitro drug release swelling biodegradation
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《医学前沿(英文)》 2017年 第11卷 第2期 页码 247-252 doi: 10.1007/s11684-017-0519-6
The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.
关键词: in vitro fertilization PCOS free radical embryo quality
John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 340-350 doi: 10.15302/J-FASE-2018209
Interest in understanding the digestion behavior of starch in the presence of dietary fibers is growing due to the ability of dietary component to control the release and absorption of glucose. This presents an outstanding opportunity to improve the quality of food products by incorporating dietary fiber into starchy food products. The physicochemical properties of different fibers and their behavior in the gastrointestinal tract (GIT) differ. To test the efficacy of these different fibers on starch digestion, static in vitro digestion models under conditions that mimic the human GIT are frequently used. Indeed, many efforts have been committed to the development of various static in vitro protocols for starch digestion. Though not considered as the gold standard in digestibility studies in food science and technology, static simulated models provide a useful alternative to in vivo techniques for rapid screening of the digestibility of food products under conditions that simulate the human GIT. This review presents the current status and development of digestion techniques for simulating digestion conditions in the human GIT, with particular interest on starch digestion in the presence of dietary fiber in the three phases of digestions including the oral, gastric and the intestinal steps. This summary can benefit investigators in developing static in vitro digestion models designed to simulate starch digestion with relevant values of the quantifiable parameters, including pH, enzymes and simulated digestive fluids.
关键词: dietary fiber in vitro digestion nutrition simulation starch
谢兰,刘冉,冯娟,邢婉丽,程京
《中国工程科学》 2017年 第19卷 第2期 页码 29-36 doi: 10.15302/J-SSCAE-2017.02.005
分子诊断是个体化医学的重要组成部分,已成为体外诊断行业的主要增长动力。近年来,尽管我国的分子诊断市场规模较小,但增长迅速。本研究通过文献研究、专家研讨、实地考察等方式,对我国的分子诊断行业进行市场分析,研究我国分子诊断行业的现状及特点,理清该产业在创新、产业转化及相关政策法规上面临的机遇和挑战,提出克服现有管理体制制约的对策建议,对推动产业健康发展具有重要的意义。
Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris
《医学前沿(英文)》 2019年 第13卷 第6期 页码 627-638 doi: 10.1007/s11684-019-0697-5
关键词: artificial ovary artificial uterus artificial gametes assisted reproduction considerations in vitro fertilization
Effects of red and blue LEDs on
Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU
《农业科学与工程前沿(英文)》 2018年 第5卷 第2期 页码 197-205 doi: 10.15302/J-FASE-2018224
关键词: blue LED microtuber plant tissue culture potato plantlets in vitro red LED
余宏亮,常明秀,曹恒海,薄立伟,杨永俊,李聪敏,李铭
《中国工程科学》 2015年 第17卷 第6期 页码 86-88
目的:项目拟通过观察在培养液中加入较高剂量的人参皂苷CK(Ginsenoside,G组)、淫羊藿苷(Icraiin,I组)两种药物前﹑后精子死亡率数据,评估两种药物单体在较大剂量时对体外精子的死亡率的影响。方法:新鲜液化的精液(semen)标本40份,F-10培养液调整浓度为1.0×107/mL,分别添加不同剂量的人参皂苷CK(Ginsenoside,Gin CK)﹑淫羊藿苷(Icraiin,Icr)按照不同药物浓度,G组分为500 μg/mL组(G1组),100 μg/mL组(G2组),I组分为500 μg/mL(I1组),100 μg/mL(I2组);设不添加任何物质的F-10组(F-10,F组)为阴性对照组,培养24 h后分别检查各组精子活动率和死亡率。结果:培养24 h后,精子死亡率从低到高分别是培养24 h后,I2组死亡率为41.31±17.49, F组死亡率为47.50±19.85 ,G2组死亡率为55.75±24.93,I3组死亡率为 58.05±19.06,G1组死亡率为98.84±1.62。精子死亡率从低到高分别是I2组﹑F组﹑G2组﹑I1组﹑G1组,各组比较有差异(P<0.05)。结论:较大剂量的Gin CK、Icr,均可引起体外培养的精子死亡率增高;在0.1 mg/mL的浓度下的Gin CK对精子的致死作用已经比较明显,而0.5 mg/mL对体外培养精子来说是致死剂量(98.84 %),在0.1 mg/mL的剂量下的淫羊藿苷对精子的致死作用并不明显,在0.5 mg/mL剂量下Icr对精子致死作用有所提高,但远未达到Gin CK 98.4%的致死率。
LIU Cao,XU Yiping,MA Mei,HUANG Bingbin,WU Jingdong,MENG Qingyi,WANG Zijian,GEARHEART Robert Alan
《环境科学与工程前沿(英文)》 2014年 第8卷 第3期 页码 433-440 doi: 10.1007/s11783-013-0594-0
关键词: sewage sludge in vitro bioassay endocrine disruption dioxin-like effect
Scale study of sites and pavilions for World Expo 2010
ZHOU Zhuoyan, CHEN Yi
《结构与土木工程前沿(英文)》 2008年 第2卷 第1期 页码 102-106 doi: 10.1007/s11709-008-0012-z
关键词: pavilion study number appropriate design
标题 作者 时间 类型 操作
Antimicrobial activity of topical agents against Propionibacterium acnes: an in vitro study of clinical
null
期刊论文
In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water
Kaifeng RAO, Na LI, Mei MA, Zijian WANG
期刊论文
and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro
null
期刊论文
extract and Ginkgolide B against oxygen–glucose deprivation/reoxygenation and glucose injury in a new in vitro
null
期刊论文
Biodegradable polymethacrylic acid grafted psyllium for controlled drug delivery systems
Ranvijay KUMAR, Kaushlendra SHARMA
期刊论文
Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality
null
期刊论文
Implications of static
John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG
期刊论文
What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations
Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris
期刊论文
Effects of red and blue LEDs on
Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU
期刊论文
Evaluation of endocrine disruption and dioxin-like effects of organic extracts from sewage sludge in autumn in Beijing, China
LIU Cao,XU Yiping,MA Mei,HUANG Bingbin,WU Jingdong,MENG Qingyi,WANG Zijian,GEARHEART Robert Alan
期刊论文